The Australian Stem Cell Centre (ASCC) has delivered intensive hands-on human Embryonic Stem Cell (hESC) training workshops since 2006. Recently these workshops have also included instructions in induced Pluripotent Stem Cell (iPSC) technology.
To date, over 40 registrants have successfully completed the training course. Course participants have included academic and biotechnology company researchers, post-graduate students, technologists and clinicians from Australia and abroad (New Zealand, Turkey, Saudi Arabia, Pakistan, India, Malaysia).
The workshops employ an intensive hands on, laboratory-based approach to ensure that all participants have the opportunity to master techniques and witness the outcome of their efforts. Generally a maximum of four trainees are enrolled in each course to ensure efficient and effective information transfer while maximising the opportunity for one-on-one interaction with the faculty.
In addition, following the course, the participants can also contact course tutors for follow-up assistance when needed. The Workshops are run in state-of-the-art dedicated hESC-laboratories at the ASCC Core hESC Laboratories facilities at both locations.
Prerequisites
To be eligible to apply for a basic stem cell training course, the participants must have had at least the equivalent of one month of general hands-on tissue culture experience and a basic understanding of the principles of cell culture, obtained either through work experience or relevant training and education.
For more details, see the below brochure:
Training Workshops Brochure - Detailed Overview
Melbourne Workshop - November 22 - 26 2010
To express interest and inquire about the next workshops to be organised in 2010, please contact us.
Examples of the lectures and practicals covered in recent courses:
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LECTURES
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PRACTICALS
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Laboratory Introduction
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Preparation of Mitomycin C Treated Mouse Embryonic Fibroblast Feeder Layers
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Aseptic Techniques in the Cell Culture
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Mouse Embryonic Fibroblast Cryopreservation
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Embryonic Stem Cells: From Derivation to Delivery & induced Pluripotent Stem (iPS) cells
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Preparation of Matrigel coated tissue culture flasks
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Basic Principles of Human Embryonic Stem and iPS Cell Culture
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Thawing Mouse Embryonic Fibroblasts
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The Cell Culture Microenvironment
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Manual Passaging of Human Embryonic Stem Cells
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Maintenance of hESC and iPSC in Serum-Free and Xeno-Free Conditions
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Freezing and Thawing of Human Embryonic Stem Cells
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Human Embryonic Stem Cell Differentiation
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Enzymatic Passaging of Human Embryonic Stem Cells
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Introduction to Flow Cytometry
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Feeder Free Culture of Human Embryonic Stem Cells & iPS cells
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Round Table Discussion
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Flow Cytometric Analysis of Human Embryonic Stem Cells
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Refund policy: ASCC will provide the full fee less AUD$50 administration
fee (+GST) up to one month prior to the course and thereafter no
refund, however the place can be transferred to another person if the
course participant locates a replacement for themselves.
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