Module 8: Characterisation and Utilisation of iPS Cells for Dental Regeneration

Module Leaders Professor Mark Bartold

Professor Stan Gronthos
Host Organisations Colgate Australian Clinical Dental Research Centre, University of Adelaide

Institute  of Medical and Veterinary Science/Hanson Institute, Centre for Stem Cell Research

Module description

Generating iPS cells from dental cells represents a major step forward in developing a successful cell based treatment for periodontitis and peri-implantitis. It may eventually allow the creation of an unlimited supply of immuno-compatible progenitor cells directly from patients’ somatic cells.

Furthermore, characterisation and functional analysis of iPS cells and their derivatives will help to identify novel components and pathways that mediate periodontal regeneration.

Photo, module members (left to right): Peter Zilm, Danijella Menicanin, Krzysztof Mrozik, Stan Gronthos, Mark Bartold, Victor Marino, Naohisa Wada

Aims

Hypothesis

Cells derived from gingival tissue will show a greater potential for iPS production then current sources and these cells can be used as a source of pluripotent cells for dental tissue regeneration.

Specific aims

  1. To determine which cells from oral sites allow for ease and efficiency of iPS generation.
  2. To use the most efficient source of oral cells and investigate their utility for tissue regeneration around teeth and dental implants.

Module Leader biographies

Professor Mark Bartold has held university positions in both Australia and the USA and is currently Professor and Director of the Colgate Australian Clinical Dental Research Centre. From 1992 – 2002 he was Professor of Periodontics at the University of Queensland. He is a member of the editorial board of eight international dental journals, an Associate Editor of the Journal of Periodontal Research and Editor of the Australian Dental Journal.

Professor Bartold is a past president of many professional organisations including the ANZ Division of the IADR, the Asian Pacific Society of Periodontology, the IADR Periodontal Research Group and the Division of Periodontics of the Royal Australasian College of Dental Surgeons. He is currently Chairman of the Board of the Australian Periodontology Research Foundation, and a Board member of the International Academy of Periodontology.


In 2004 Professor Bartold was awarded Member of the Order of Australia for service to the dental profession, particularly through periodontic education and research and the establishment of the Australian Periodontal Research Foundation.


Professor Stan Gronthos completed his PhD in 1999, and undertook a research fellowship at the prestigious National Institutes of Health (1999-2001), Bethesda, Maryland, USA. In 2002, Professor Gronthos returned to Adelaide on a RAH Florey Fellowship where he founded the Mesenchymal Stem Cell Group within the Division of Haematology, IMVS/Hanson Institute. In 2006, he was appointed Head of the Regenerative Medicine Program within the Division of Haematology, IMVS.

To date, Professor Gronthos has co-authored 67 peer reviewed publications and several book chapters with a total of 3,490 citations. He is  on the Editorial Board of for the Journal of Stem Cell Research and the journal of Stem Cells.

Since 2005 Professor Gronthos has been a scientific consultant to Angioblast Systems Inc. New York, and its sister company Mesoblast Ltd in Melbourne, whom have exclusive licensing rights to his stem cell patent family. The research is on track to facilitate a successful industry/commercial translation in the area of regenerative medicine.

Contact details

Professor Mark Bartold

 E-mail   mark.bartold@adelaide.edu.au
 Phone   +61 8 8303 3437
 Web
  http://health.adelaide.edu.au/dentistry/colgate/research/

Professor Stan Gronthos

 E-mail  
 stan.gronthos@imvs.sa.gov.au 
 Phone  +61 8 8222 5350
 Web  www.hansoninstitute.sa.gov.au/aboutus/index.php

Selected publications

  1. Gronthos S, Ohta S, Graves SE, Simmons PJ (1994). The STRO-1+ fraction of adult human bone marrow contains the osteogenic precursors. BLOOD, 84 (12):4164-4173. IF=10.9, (231).
  2. Gronthos S, and Simmons PJ (1995). The growth factor requirements of STRO-1+ human bone marrow stromal precursors under serum-deprived conditions. BLOOD, 85 (4): 929-940. IF=10.9, (145).
  3. Gronthos S, Mankani M, Brahim J, Gehron Robey P, Shi S (2000). Post-Natal Human Dental Pulp Stem Cells In vitro and In vivo. Proceedings of the National Academy of Sciences (USA), 97 (25): 13625-13630. IF=9.6, (290).
  4. Kuznetsov SA, Mankani MH, Gronthos S, Satomura K, Bianco P, Gehron Robey P (2001). Circulating skeletal stem cells. Journal of Cell Biology, 153 (5):1133-1139. IF=9.6, (223).
  5. Gronthos S, Dawn M, Franklin DM, Holly A, Leddy HA, Gehron Robey P,Storms RW, and Gimble JM (2001). Surface Protein Characterization of Human Adipose Tissue-Derived Stromal Cells. Journal of Cellular Physiology 189: 54-63.IF=3.6, (192).
  6. Ivanovski S, Haase HR, Bartold PM. Isolation and characterization of fibroblasts derived from regenerating periodontal defects. Archives of Oral Biology 46: 679-688; 2001.
  7. Ivanovski S, Haase HR, and Bartold PM. Characterization of regenerative phenotypes of primary and cloned cultures of human periodontal fibroblasts. Journal of Dental Research 80: 1665-1671; 2001.
  8. Seo B-M, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J, Young M, Robey PG, Wang C-Y, Shi S. Multipotent postnatal stem cells from human periodontal ligament. Lancet 364:149-155; 2004
  9. Shi, S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthopedics and Craniofacial Research 8: 191-199; 2005
  10. PM Bartold, S Shi, S Gronthos Stem Cells and Periodontal Regeneration Periodontology 2000 40: 164-172; 2006